U z o h , R a y mo n d D .
A k a n d e , E . K .
M a n u , M . A .
Department of Science Laboratory Technology
Although the extraction of bioactive agents from plants has been one of the most intensive areas of natural products research, a number of medicinal plants remain unexplored. The pulverized seeds of citrulus colocynthis (Water Melon) was extracted with n-hexane, petroleum ether and water. The oil extract tested positive for alkaloid, tannin, phlobatannin, phenol, flavannoid, and cardiac glycoside. Water extract tested positive for alkaloid, tannin, phlobatannin, phenol,saponins, and negative for flavonoids, and glycoside in the water extract. Antimicrobial assay with staphylococcus aureus and Escherichia coli at 0.25cm3 ,0.5cm3 ,1cm3 , and 1.5cm3 concentrations produced moderate inhibition of the growth of the test organisms, while in the antimicrobial disc method, only the water extract produced significant zone of inhibition of 0.01mm and 0.02mm respectively. The zone of inhibition of the seeds extracts under study was below 10mm, the recommended value of inhibition for an antibiotic pending further research.
Keywords: Bioactive Agents, Phytochemicals, Antibiotic, Antimicrobial, Natural Products.
International Journal of Food and Agricultural Research Vol.14 No. 1&2 © 2019 by The Development Universal Consortia. All Rights Reserved